Skip to main content

CoronaVac-inactivated COVID-19 vaccine

 CoronaVac is an inactivated vaccine candidate against COVID-19, created from African green monkey kidney cells (Vero cells) that have been inoculated with SARS-CoV-2 (CN02 strain). 

At the end of the incubation period, the virus is harvested, inactivated with β-propiolactone (BPL), concentrated, purified, and finally absorbed onto aluminium hydroxide. The aluminium hydroxide complex is then diluted in a sodium chloride, phosphate-buffered saline, and water solution before being sterilised and filtered ready for injection. 

Vaccine is prepared in a Good Manufacturing Practice-accredited facility of Sinovac Life Sciences (Beijing, China) that is periodically inspected by the Chinese National Medical Products Administration committee for compliance. 

Vaccine of 3 μg in 0·5 mL of aluminium hydroxide diluent per dose in ready-to-use syringes is administered intramuscularly according to the dosing schedule of either day 0 and day 14, or day 0 and day 28, depending on the cohort.


BPL


BPL is widely used for the inactivation of infectious agents (bacteria, fungi, viruses) in many vaccine preparations as well as in disinfection, plasma sterilization and tissue transplants

BPL reacts with nucleophilic reagents (including nucleic acids and proteins) leading to alkylated and/or acylated products. BPL is very reactive with different chemical moieties of various biological molecule including proteins (mostly methionine, cysteine and histidine) and nucleic acids (mainly adenosine, cytidine and guanosine moieties of the vRNA)

Ref:

1. Safety, tolerability, and immunogenicity of an inactivated SARS-CoV-2 vaccine in healthy adults aged 18–59 years: a randomised, double-blind, placebo-controlled, phase 1/2 clinical trial

Zhang, Yanjun et al. The Lancet Infectious Diseases, Volume 0, Issue 0


2. Pierre Bonnafous, Marie-Claire Nicolaï, Jean-Christophe Taveau, Michel Chevalier, Fabienne Barrière, Julie Medina, Olivier Le Bihan, Olivier Adam, Frédéric Ronzon, Olivier Lambert,
Treatment of influenza virus with Beta-propiolactone alters viral membrane fusion,
Biochimica et Biophysica Acta (BBA) - Biomembranes,
Volume 1838, Issue 1, Part B, 2014, Pages 355-363

Comments

Post a Comment

Popular posts from this blog

Mounjaro, Is it a game changer for weight loss and diabetes?

  MOUNJARO™ ( Tirzepatide) was approved for medical use in the United States in May 2022 WEIGHT LOSS: (Not approved by FDA for weight loss) Eli Lilly and Company is  finalizing an application to the U.S. Food and Drug Administration for fast-track approval to sell tirzepatide for chronic weight management. MOUNJARO™ is a glucose-dependent insulinotropic polypeptide (GIP) receptor and glucagon-like peptide-1 (GLP-1) receptor agonist indicated in adults for type 2 diabetes mellitus management. CONTRAINDICATIONS Personal or family history of medullary thyroid carcinoma or in patients with Multiple Endocrine Neoplasia syndrome type 2  Known serious all to tirzepatide or any of the excipients in MOUNJARO                                                                    ...
Post a Comment
Read more

Thyroid health- Quality of Life

  Let's get a TSH blood test for our family and friends Normal serum TSH ranges are higher in the  people age 70 to 80 years ; based on this, higher serum TSH goals may be needed as a patient ages.  The American Thyroid Association (ATA) suggests raising the target serum TSH to 4-6 m IU/L for 70 years-old and up The thyroid is a small, butterfly-shaped hormone-producing gland located at the front of the neck. The thyroid gland secretes two main hormones:  thyroxine (T-4) and triiodothyronine (T-3).   These hormones regulate energy levels, metabolism of fats, proteins and carbohydrates, body temperature, heart rate.  Hypothyroidism happens when the underactive thyroid gland doesn't make enough hormones. Hypothyroidism affects up to 10% of the general population (5% undiagnosed) . A n autoimmune disease called Hashimoto's diseas e where our body  makes antibodies that attack healthy thyroid tissues  is the most common cause of hypothyroidism in soc...
Post a Comment
Read more

Vaccines, XBB.1.5 variant and antibody evasion

Corona virus XBB and XBB.1 were first identified in India in mid-August and quickly became predominant in India.  The spike protein of the predominant XBB subvariant has 14 mutations in addition to those found in BA.2, including 5 in the N-terminal domain (NTD) and 9 in the receptor-binding domain (RBD), whereas XBB.1 has an additional G252V mutation. Single and combination therapies with monoclonal antibodies have received emergency use authorization for COVID-19.  Using  COVID-19 pseudovirus, which enables the study of the SARS-CoV-2 virus in cell culture,   XBB, and XBB.1 subvariants were found to have far greater antibody resistance than earlier variants. XBB sublineages are completely resistant to bebtelovimab, leaving us with no authorized antibody for treatment use. The combination of mAbs known as Evusheld that is authorized for the prevention of COVID-19 is also completely inactive against the new subvariants. This poses a serious problem for millions of im...
Post a Comment
Read more